The sperm is examined in the andrology laboratory and if it is of suitable quality, it is prepared for freezing under special conditions. The freezing medium is added to the washed sperm, and in the next step, the sperm sample is poured into straws for freezing and placed on liquid nitrogen steam for 15-30 minutes. Finally, the freezing straws are immersed in liquid nitrogen and the temperature of sperm cells or testicular tissue is reduced to -196 degrees Celsius in liquid nitrogen or LN2 (Liquid Nitrogen). The frozen cells are stored in the sperm bank. Frozen sperm can survive for years and then be used in assisted reproduction methods. The survival of the cell after freezing depends on how the two processes of freezing and thawing are performed.